Preliminary Assessment of the Safety and Immunogenicity of a New CTXF-Negative, Hemagglutinin/Protease-Defective El Tor Strain as a Cholera Vaccine Candidate
نویسندگان
چکیده
1999, 67(2):539. Infect. Immun. and Gustavo Sierra Talena Ledón, Manuel Díaz Jidy, Miriam Lastre, Laura Bravo Valmaseda, Oliver Pérez, Alberto Pérez, Margarita Ramírez, Campos, Boris L. Rodríguez, JoséL. Pérez, Tania Rafael Fando, Barbara Cedré, Antonio Pérez, Javier Jorge A. Benítez, Luis García, Anisia Silva, Hilda García, Strain as a Cholera Vaccine Candidate Hemagglutinin/Protease-Defective El Tor -Negative, Φ Immunogenicity of a New CTX Preliminary Assessment of the Safety and
منابع مشابه
Preliminary assessment of the safety and immunogenicity of a new CTXPhi-negative, hemagglutinin/protease-defective El Tor strain as a cholera vaccine candidate.
Vibrio cholerae 638 (El Tor, Ogawa), a new CTXPhi-negative hemagglutinin/protease-defective strain that is a cholera vaccine candidate, was examined for safety and immunogenicity in healthy adult volunteers. In a double-blind placebo-controlled study, no significant adverse reactions were observed in volunteers ingesting strain 638. Four volunteers of 42 who ingested strain 638 and 1 of 14 who ...
متن کاملConstruction and characterization of a nonproliferative El Tor cholera vaccine candidate derived from strain 638.
In recent clinical assays, our cholera vaccine candidate strain, Vibrio cholerae 638 El Tor Ogawa, was well tolerated and immunogenic in Cuban volunteers. In this work we describe the construction of 638T, a thymidine auxotrophic version of improved environmental biosafety. In so doing, the thyA gene from V. cholerae was cloned, sequenced, mutated in vitro, and used to replace the wild-type all...
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Peru-15 is a live attenuated oral vaccine derived from a Vibrio cholerae O1 El Tor Inaba strain by a series of deletions and modifications, including deletion of the entire CT genetic element. Peru-15 is also a stable, motility-defective strain and is unable to recombine with homologous DNA. We wished to determine whether a single oral dose of Peru-15 was safe and immunogenic and whether it wou...
متن کاملDetermination of ctxAB expression in Vibrio cholerae Classical and El Tor strains using Real-Time PCR
Cholera is an infection of the small intestines caused by the bacterium V. cholerae. It is a major cause of health threat and also a major cause of death worldwide and especially in developing countries. The major virulence factor produced by V. cholerae during infection is the cholera toxin. Total mRNA extraction and reverse transcription was performed for making ctxAB cDNA. Relative Real-Time...
متن کاملProlonged colonization of mice by Vibrio cholerae El Tor O1 depends on accessory toxins.
Cholera epidemics caused by Vibrio cholerae El Tor O1 strains are typified by a large number of asymptomatic carriers who excrete vibrios but do not develop diarrhea. This carriage state was important for the spread of the seventh cholera pandemic as the bacterium was mobilized geographically, allowing the global dispersion of this less virulent strain. Virulence factors associated with the dev...
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